A glycan array screen revealed Nesd affinity for β-galactosides. His-tagged Nesd was expressed in a baculovirus system and used to screen an array of 320 glycans (version 3.0) spotted in six replicates. Nesd binding was detected first using an anti-His rabbit antibody and then an anti–rabbit IgG Alexa Fluor 488. The experiment was replicated twice with very similar results, and complete sets of data are available at the CFG website. The graph here shows the results of one of these experiments in which each glycan was plotted against its mean relative fluorescence intensity. The top high affinity ligands are indicated on the chart, and β-galactose residues are highlighted in red. The error bars indicate standard deviations.