Distinct targeting mechanisms impart distinct Myo1 localization dynamics during the cell cycle. (A) Effects of bni5Δ and myo1 truncations on the dynamics of Myo1 localization during the cell cycle. Cells of strains XDY286 (MYO1-GFP; n = 33), XDY287 (MYO1-GFP bni5Δ; n = 38), XDY288 (myo1-Tail-GFP; n = 88), XDY289 (myo1-TD2Δ-GFP; n = 42), and XDY290 (myo1-TD2-GFP; n = 31) were grown in YPD at 23°C and then imaged by 3D dual-color time-lapse microscopy at 23°C with a 1-min interval. Montage images from the representative time-lapse data are shown here. Top row in each image panel: Cdc3-RFP (red); middle row: Myo1*-GFP (asterisk indicates Myo1 or its fragment; green); bottom row: merged images of septin-RFP and Myo1*-GFP. The merged images were used to determine the symmetry of Myo1*-GFP ring during its localization and/or constriction. Arrowheads indicate septin-hourglass splitting. Images within each image panel were processed with the same magnification. (B) Constriction rates of Myo1 and its fragments in wild-type and/or bni5Δ cells. “a” indicates that only cells showing symmetric Myo1 constriction were used for the calculation.