Lte1 binds to and negatively regulates Bud1. (a) Cells expressing Lte1-GFP (MGY518), or Lte1-8N-GFP (MGY517) and Ras2, or cells expressing Lte1-8N^R1343E-GFP (MGY527) alone under GAL1 promoter control were cultivated in galactose- or glucose-containing medium. Budding scars were visualized by Calcofluor staining. Bar, 5 µm. (b) WT (MGY308), lte1Δ (MGY309), and lte1Δ bud1Δ (MGY498) cells, expressing Kel1-GFP and Spa2-GFP, were treated with nocodazole for 2.5 h. Bar, 5 µm. (c) Quantification of the cells in b (n > 150). Error bars show the SD of three independent experiments. (d) Haploid (MGY309) and diploid (MGY485) lte1Δ cells, expressing Kel1-GFP and Spa2-GFP, were treated with nocodazole for 2.5 h as in b. Percentages of cells with a proximal, distal, or dispersed polarizome are indicated (n > 100). Error bars show the SD of three independent experiments. Bar, 5 µm. (e) lte1Δ bud3Δ cells expressing Kel1-CFP (MGY603) were treated with nocodazole for 2.5 h as in b. Bar, 5 µm.