lte1Δ mutants arrested in mitosis have a polarized bud. (a) Wild-type (WT; 15D) and lte1Δ cells (SY144) were arrested in nocodazole for 2 h; percentage of “hammerhead” phenotype is reported (n > 250). Bar, 5 µm. (b) Localization of Kel1-GFP in WT (SY159), lte1Δ (SY160), and lte1Δ cells complemented by Lte1, Lte1^K1273E, or Lte1-Cdk arrested in nocodazole for 2 h. Bar, 5 µm. (c) WT (15D) and lte1Δ (SY144) cells were arrested in α-factor and released in medium containing nocodazole. At the indicated time, cells were harvested and Clb2 levels (top panel) and H1-associated kinase activity (bottom panel) were assayed. (d) Kel1-GFP localization in swe1Δ (MGY340) and swe1Δ lte1Δ (MGY341) cells arrested in nocodazole for 2 h. Bar, 5 µm.