Pcdh19 does not mediate significant calcium-dependent bead aggregation. (A and B) Beads coated with NcadEC-Fc in the absence (A) or presence (B) of calcium. (C) Distribution of NcadEC-Fc–coated bead aggregate sizes in the absence and presence of calcium. (D and E) Beads coated with Pcdh19EC-Fc in the absence (D) or presence (E) of calcium. Bar, 100 µm. (F) Distribution of Pcdh19EC-Fc–coated bead aggregate sizes in the absence and presence of calcium.