Overexpression of Pcdh19 or Ncad fails to reciprocally rescue. Cumulative probability distributions of neural plate widths in five-somite stage embryos. (A and B) Expression of Pcdh19-GFP (A) or Ncad-GFP (B) does not affect neural plate width. (C and D) Expression of Pcdh19-GFP (C) or Ncad-GFP (D) can compensate for the loss of endogenous Pcdh19 or Ncad, respectively. (E and F) Expression of Pcdh19-GFP (E) or Ncad-GFP (F) cannot reciprocally rescue ncad morphants or pcdh19 morphants, respectively. HS, heat shocked; Un-HS, un-heat shocked.