Figure 8. PI(3)P controls the... | Rockefeller University Press

Figure 8.

$Figure 8. PI(3)P controls the association of Inpp5B with phagosomes. (A) Cells were transiently transfected with Inpp5B-GFP, and phagocytosis of complement-coated RBCs was initiated. Shortly after ingestion of the RBCs, the cells were treated with 100 µM LY294002 (middle) or 100 nM PI-103 (bottom). Numbers indicate time in seconds after phagosome formation, and arrows point to the nascent or recently formed phagosomes. Bars, 5 µm. (B) Quantitation of the percentage of phagosomes that was Inpp5B-GFP positive at 10 min after phagosome sealing. Data are means ± SEM of at least three individual experiments; ≥30 phagosomes were counted. The numbers indicate the fraction of Inpp5B-positive phagosomes. (C) Plots of the background-subtracted intensity of Inpp5B-GFP for phagosomes under control conditions and when 100 µM LY294002 (arrowhead) was added shortly after phagosome closure (squares). Data are means ± SEM of at least three individual experiments; ≥10 phagosomes were counted per experiment. a.u., arbitrary units.$

PI(3)P controls the association of Inpp5B with phagosomes. (A) Cells were transiently transfected with Inpp5B-GFP, and phagocytosis of complement-coated RBCs was initiated. Shortly after ingestion of the RBCs, the cells were treated with 100 µM LY294002 (middle) or 100 nM PI-103 (bottom). Numbers indicate time in seconds after phagosome formation, and arrows point to the nascent or recently formed phagosomes. Bars, 5 µm. (B) Quantitation of the percentage of phagosomes that was Inpp5B-GFP positive at 10 min after phagosome sealing. Data are means ± SEM of at least three individual experiments; ≥30 phagosomes were counted. The numbers indicate the fraction of Inpp5B-positive phagosomes. (C) Plots of the background-subtracted intensity of Inpp5B-GFP for phagosomes under control conditions and when 100 µM LY294002 (arrowhead) was added shortly after phagosome closure (squares). Data are means ± SEM of at least three individual experiments; ≥10 phagosomes were counted per experiment. a.u., arbitrary units.