Figure 1. Identification and... | Rockefeller University Press

Figure 1.

$Figure 1. Identification and quantification of intermediates in platelet release by immunofluorescence microscopy. The released proplatelet-enriched fraction and washed mouse platelets (control) were probed with a rabbit polyclonal antibody against detyrosinated tubulin and analyzed by immunofluorescence microscopy for different-sized objects. Intermediates in platelet release were categorized based on perimeter (proplatelets) or diameter (megakaryocytes, preplatelets, and platelets). (A) Representative pictures of a day 5 proplatelet-enriched culture before (left) and after (middle) thresholding of anti-tubulin–labeled intermediates using MetaMorph software and washed mouse platelets (0.5–2-µm diameter; right). (B) Relative distribution of intermediates at day 5 of culture after proplatelet enrichment. (C) Representative pictures of the tubulin cytoskeleton of mouse preplatelets (2–10-µm diameter; immunofluorescence microscopy; inset), their ultrastructure (thin-section EM; bottom), and cytoskeleton (rapid-freeze EM; top).$

Identification and quantification of intermediates in platelet release by immunofluorescence microscopy. The released proplatelet-enriched fraction and washed mouse platelets (control) were probed with a rabbit polyclonal antibody against detyrosinated tubulin and analyzed by immunofluorescence microscopy for different-sized objects. Intermediates in platelet release were categorized based on perimeter (proplatelets) or diameter (megakaryocytes, preplatelets, and platelets). (A) Representative pictures of a day 5 proplatelet-enriched culture before (left) and after (middle) thresholding of anti-tubulin–labeled intermediates using MetaMorph software and washed mouse platelets (0.5–2-µm diameter; right). (B) Relative distribution of intermediates at day 5 of culture after proplatelet enrichment. (C) Representative pictures of the tubulin cytoskeleton of mouse preplatelets (2–10-µm diameter; immunofluorescence microscopy; inset), their ultrastructure (thin-section EM; bottom), and cytoskeleton (rapid-freeze EM; top).