Figure 5.
Figure 5. SEPT9 mediates exocyst complex localization to the midbody. (A) Quantification of the effect of SEPT9 KD on the localization of midbody components. The percentage of cells exhibiting enrichment of the protein of interest at the midbody was determined by immunofluorescence microscopy after control or SEPT9 KD. Data are represented as mean ± SEM (error bars; n ≥ 300 cells from three independent experiments). Asterisks indicate differences between control and SEPT9 KD cells; **, P < 0.001. (B and C) Representative example of the localization of the exocyst component Sec8 in cytokinetic cells upon control (B) or SEPT9 KD (C). Arrows point to the midbody. (D) Sec8 fluorescence at the midbody was quantified using ImageJ upon control or SEPT9 KD; **, P < 0.0001. (E) Double staining of SEPT9 and Sec8 in cytokinetic cells. Bars, 17 µm.

SEPT9 mediates exocyst complex localization to the midbody. (A) Quantification of the effect of SEPT9 KD on the localization of midbody components. The percentage of cells exhibiting enrichment of the protein of interest at the midbody was determined by immunofluorescence microscopy after control or SEPT9 KD. Data are represented as mean ± SEM (error bars; n ≥ 300 cells from three independent experiments). Asterisks indicate differences between control and SEPT9 KD cells; **, P < 0.001. (B and C) Representative example of the localization of the exocyst component Sec8 in cytokinetic cells upon control (B) or SEPT9 KD (C). Arrows point to the midbody. (D) Sec8 fluorescence at the midbody was quantified using ImageJ upon control or SEPT9 KD; **, P < 0.0001. (E) Double staining of SEPT9 and Sec8 in cytokinetic cells. Bars, 17 µm.

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