Figure 2.
Figure 2. Roles of individual septins in cell division. (A) HeLa cells were treated with the indicated siRNA, and lysates were probed as specified. Numbers to the right of the blot represent molecular mass standards in kilodaltons. (B) Representative examples of cells treated with control siRNA, and of multinucleated cells and cells attached by persistent midbodies after SEPT9 KD (red, α-tubulin; blue, DNA). Bars, 17 µm. (C) Quantification of effect of septin KD on cytokinesis. The percentage of cells exhibiting multinucleation or midbody attachment was determined upon treatment with the indicated siRNA. Data are represented as mean ± SEM (error bars; n ≥ 300 cells from three or more independent experiments). Asterisks indicate differences between control and septin KD cells. *, P < 0.01; **, P < 0.0001.

Roles of individual septins in cell division. (A) HeLa cells were treated with the indicated siRNA, and lysates were probed as specified. Numbers to the right of the blot represent molecular mass standards in kilodaltons. (B) Representative examples of cells treated with control siRNA, and of multinucleated cells and cells attached by persistent midbodies after SEPT9 KD (red, α-tubulin; blue, DNA). Bars, 17 µm. (C) Quantification of effect of septin KD on cytokinesis. The percentage of cells exhibiting multinucleation or midbody attachment was determined upon treatment with the indicated siRNA. Data are represented as mean ± SEM (error bars; n ≥ 300 cells from three or more independent experiments). Asterisks indicate differences between control and septin KD cells. *, P < 0.01; **, P < 0.0001.

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