Determinants of SRGP-1 localization. (A) SRGP-1::GFP retains its junctional localization upon hmr-1/cadherin depletion via RNAi. (B) SRGP-1::GFP localizes to junctions in embryos homozygous for a null allele of sax-3/Robo, ky123. (C) Deletion of the F-BAR domain abolishes SRGP-1 junctional localization in the early embryo, but later, SRGP-1(ΔF-BAR) is recruited to epidermal AJs during elongation. (D) The F-BAR domain alone distributes uniformly over the entire PM. (E) A 200-aa sequence beyond the F-BAR domain is sufficient to confer junctional localization at all developmental stages. (F) The GAP domain is dispensable for SRGP-1 localization. (G) Deletion of the C terminus of SRGP-1 results in largely wild-type distribution, with some aggregates.