SRGP-1 localizes to cell–cell junctions in C. elegans embryos. (A) Nomarski and confocal projections of SRGP-1::GFP demonstrate localization at cell–cell contacts throughout embryogenesis. (B) SRGP-1::mCherry and HMP-1::GFP in an elongating embryo show colocalization of the two proteins at epidermal adherens junctions. (C) SRGP-1::GFP and DLG-1::dsRed in an elongating embryo show partial colocalization, where SRGP-1 occupies a space mostly apical to DLG-1. Insets are the side views of the boxed junctions. (D) Immunolabeling of endogenous SRGP-1 with an antibody validates SRGP-1::GFP localization.