Identification of subcellular markers for in vivo imaging of zebrafish cells. Images of zebrafish Pac2 fibroblasts transfected with pCS2+ constructs encoding fluorescently tagged markers for subcellular labeling 24 h after transfection. (A) β3-tubulin-GFP, (B) GFP-tubulin, (C) EB1-GFP, (D) GFP-DCX, (E) Tau-GFP, and (F) EB3-GFP. (G) mito-DsRed to label mitochondria in red, YFP-DCX to label microtubules in yellow, memCFP to label the cytoplasmic membrane in blue and H2B-CFP to label the nucleus in blue; (H) DsRed2-ER to label the ER in red, Golgi-YFP to label the Golgi apparatus in yellow, memCFP and H2B-CFP; (I) DCX-tdTomato to label microtubules in red, actin-Citrine to label the actin cytoskeleton in yellow, memCFP, H2B-CFP; (J) Centrin2-YFP to label the centrosome in yellow (arrow is indicating the two centrioles of the centrosome) and H2B-CFP; (K) GFP-Centrin2 to label the centrosome in green (arrow is indicating the two centrioles of the centrosome); and (L) DCX-tdTomato, Centrin2-YFP, memCFP, and H2B-CFP. The inset shows a higher magnification of the centrosome at the hub of the microtubule network. These data present a collection of subcellular-targeted fluorescent proteins tested for their specificity in zebrafish cells. “mem“ represents a membrane localization signal, which consists of a plamitylation and myristinylation sequence of the human Lck kinase.