CK1α knockdown produces a stellate phenotype. (A–C) Images of representative fields of cells with no treatment (A), Scar RNAi (B), and CK1α RNAi (C). (D) Quantification of actin retrograde flow rates in GFP-actin cells with the indicated gene depleted. Retrograde flow rates were calculated from kymograph analysis of time-lapse total internal reflection fluorescence images (mean and SEM; n = 45 measurements for each condition). Bar, 20 µm.