Figure 2.
Figure 2. Cavin-1 knockdown. (A) Cavin-1 knockdown in HeLa-CAV1-mRFP cells caused an almost complete redistribution of assembled 70S-CAV1 into unassembled 8S-CAV1 as determined by sucrose velocity gradient centrifugation. (B and C) HeLa-CAV1-mRFP cells treated with control siRNA (B) or siRNA targeting cavin-1 (C) were loaded with 100 nM LysoTracker (green) for 1 h and imaged live by confocal microscopy. Cavin-1 knockdown caused loss of caveolar spots in the plasma membrane and accumulation of CAV1-mRFP in acidic, LysoTracker-positive organelles. (B and C, bottom) Insets show enlargements of boxed areas. Blots and images are representative of at least three independent experiments. Bars, 10 µm.

Cavin-1 knockdown. (A) Cavin-1 knockdown in HeLa-CAV1-mRFP cells caused an almost complete redistribution of assembled 70S-CAV1 into unassembled 8S-CAV1 as determined by sucrose velocity gradient centrifugation. (B and C) HeLa-CAV1-mRFP cells treated with control siRNA (B) or siRNA targeting cavin-1 (C) were loaded with 100 nM LysoTracker (green) for 1 h and imaged live by confocal microscopy. Cavin-1 knockdown caused loss of caveolar spots in the plasma membrane and accumulation of CAV1-mRFP in acidic, LysoTracker-positive organelles. (B and C, bottom) Insets show enlargements of boxed areas. Blots and images are representative of at least three independent experiments. Bars, 10 µm.

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