Palmitoylation-site mutations lead to Syt VII retention in the TGN. (A) NRK cells were transduced with adenovirus expressing Lamp1-RFP and Syt VII–YFP or Syt VII (C/S)–YFP and imaged by live confocal microscopy. Syt VII–YFP colocalizes with the lysosomal marker Lamp1-RFP, but Syt VII (C/S)–YFP does not. (B) NRK cells were transduced with adenovirus expressing GalT-RFP and Syt VII–YFP or Syt VII (C/S)–YFP and imaged by live confocal microscopy. Syt VII (C/S)–YFP colocalizes with the Golgi marker GalT-RFP, whereas Syt VII–YFP does not. (C) NRK cells were transduced with adenovirus expressing Syt VII (C/S)–YFP, fixed, labeled with antibodies to TGN38, and imaged by confocal microscopy. Syt VII (C/S) colocalizes with the trans-Golgi marker TGN38. Bars, 6 µm.