Schematic diagram showing molecular pathways occurring at the leading edge of migrating astrocytes after wounding. Upon wounding, integrin activation at the leading edge induces Cdc42 activation and recruitment at the wound edge (Etienne-Manneville and Hall, 2001; Osmani et al., 2006). Active Cdc42 binds and activates the Par6–aPKC complex, which in turn induces APC and Dlg1 recruitment to leading edge microtubules to promote centrosome reorientation (Etienne-Manneville and Hall, 2003; Etienne-Manneville et al., 2005). We show here that Dlg1 regulates dynein interaction with leading edge microtubules via GKAP and subsequent microtubule polarization and cell migration.