Dlg1 controls GKAP recruitment to leading edge microtubules downstream of Cdc42. (A) TIRF images showing Dlg1 (red) and GKAP (green). (B) Cdc42 or Dlg1 depletion inhibits GKAP recruitment to the cell leading edge. Immunofluorescence of GKAP after treatment with si Cdc42 or si Dlg1 (left) and corresponding quantification. Results are shown as mean ± SEM of 300 cells from three independent experiments. Arrows point to the centrosome. Dotted lines show cell edges. (C) TIRF images of GKAP or GKAP (red) and microtubules (green) in Dlg1- or Cdc42-depleted cells. Colocalization is shown in yellow (right panels). Dashed lines indicate the orientation of the wound. Bars, 5 µm.