Aurora B provides a switch in the targeting of different dynein subunits to kinetochores. (A) Images showing the localization of the dynein light chains GFP^LAP-Tctex3 and GFP^LAP-LC8, or the dynactin subunit GFP^LAP-Arp1 in different stages of mitosis. (B) Images showing the localization of GFP^LAP-Tctex3, GFP^LAP-LC8, or GFP^LAP-Arp1 in STLC-treated cells, or STLC plus ZM447493–treated cells. (C–E) Images showing the localization of GFP^LAP-Tctex3, GFP^LAP-LC8, or GFP^LAP-Arp1 and either Mis12-INCENP(TAA)-mCherry (control) or Mis12-INCENP-mCherry–transfected cells as indicated. (F) Images showing the localization of GFP-LC8 in either control or Astrin-depleted cells. Insets show enlarged views of the boxed regions. Bars, 5 µm.