Figure 2.
![Figure 2. HURP and augmin are required for de novo interzonal MT generation in the absence of preexisting MTs. (A–D) Immunostaining of MT in control, BI2536-treated (A), GCC185-depleted (B), ZM477439-treated (C), augmin- or HURP-depleted (D) cells fixed at 5 min after the MT depolymerization/regrowth treatment. Bars, 5 µm. (A’–D’) The ratio of central and polar MT intensities of anaphase cells in A–D. Means ± SEM of three independent experiments are shown (>16 cells were analyzed for each treatment). The ratio in both the augmin- and HURP-depleted cells but not in the other cells was significantly lower (augmin and HURP [asterisks], P < 0.001; other cells, P > 0.04; by t test) than that in the control cells.](https://cdn.rupress.org/rup/content_public/journal/jcb/191/2/10.1083_jcb.201004150/10/jcb_201004150_gs_fig2.jpeg?Expires=1773456545&Signature=opvj5XVKkk3x0WX5t2WA29F1pBTuDm1jLuHsDtRRo3bb85Rgcscv6vozUD0fquhKXC9z2-HZ9E-E5LPWbZn0FnHdCdtr7KX9~QqFNCaknpfHI9HHOX9ie9zTYFQjzUkybmRzLGO4MZac7t4ImPuOON4siris3y8wIaznyoBqbJCdQKQww7g~oYAReIsQM4E5DxfMRAYy5Auu0t4DzDwjmcvNwg6WcLLVWtIh~dMxnavpCv~16WD4Pmde0oxGQjoobIaEL5rKMXU8~G5XIgycISmFz4GDUIQAcCxbzU6jb7DNTWCkv-XLEiI1OUup3FyU1qIyVO43IB3HIxrxfyrruw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HURP and augmin are required for de novo interzonal MT generation in the absence of preexisting MTs. (A–D) Immunostaining of MT in control, BI2536-treated (A), GCC185-depleted (B), ZM477439-treated (C), augmin- or HURP-depleted (D) cells fixed at 5 min after the MT depolymerization/regrowth treatment. Bars, 5 µm. (A’–D’) The ratio of central and polar MT intensities of anaphase cells in A–D. Means ± SEM of three independent experiments are shown (>16 cells were analyzed for each treatment). The ratio in both the augmin- and HURP-depleted cells but not in the other cells was significantly lower (augmin and HURP [asterisks], P < 0.001; other cells, P > 0.04; by t test) than that in the control cells.
