Cep120 is enriched at the daughter centriole. (A) NIH3T3 and RPE-1 cells were serum starved, fixed, and stained for Cep120 (green), glutamylated tubulin (red), and DNA (DAPI, blue). Note that Cep120 signal is greater on the daughter centriole. (B) NIH3T3 cells were released from serum starvation and followed over time as they progressed through the various cell cycle stages. Samples were fixed and stained for Cep120 (green), centrin (red), and DNA (blue). The asymmetric distribution of Cep120 is conserved through S phase, G2, and mitosis. (C) NIH3T3 cells were infected with lentivirus expressing Cep120-GFP. Cells were fixed and stained for GFP (green), cenexin (red), and DNA (blue). Cep120-GFP is enriched at the centrioles lacking cenexin, which corresponds to daughter centrioles at each pole. Insets are magnified images of the centrosome region. Bars, 10 µm.