Figure 1.
Figure 1. Cep120 localizes to centrioles in MTECs. (A) Western blot of lysates from human (U2OS and RPE-1) and mouse (NIH3T3, IMCD-3, and MEF) cells. Samples were probed with a polyclonal antibody against Cep120 and MAPK p38 as a loading control. Numbers on the left indicate molecular mass of markers in kilodaltons. (B) Expression levels of Cep120 in MTECs. Cells were grown on Transwell permeable filter supports, and ALI was established 2 d after cells reached confluence. Cells were harvested on the indicated days, and lysates were analyzed by Western blotting using antibodies against Cep120 and p38. (C) MTEC cultures during differentiation (see Introduction). Cells were grown on Transwell filters, fixed, and labeled with antibodies against acetylated α-tubulin or centrin (red) and Cep120 (green). Insets are magnified images of the centrosome region. Bar, 10 µm.

Cep120 localizes to centrioles in MTECs. (A) Western blot of lysates from human (U2OS and RPE-1) and mouse (NIH3T3, IMCD-3, and MEF) cells. Samples were probed with a polyclonal antibody against Cep120 and MAPK p38 as a loading control. Numbers on the left indicate molecular mass of markers in kilodaltons. (B) Expression levels of Cep120 in MTECs. Cells were grown on Transwell permeable filter supports, and ALI was established 2 d after cells reached confluence. Cells were harvested on the indicated days, and lysates were analyzed by Western blotting using antibodies against Cep120 and p38. (C) MTEC cultures during differentiation (see Introduction). Cells were grown on Transwell filters, fixed, and labeled with antibodies against acetylated α-tubulin or centrin (red) and Cep120 (green). Insets are magnified images of the centrosome region. Bar, 10 µm.

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