Wnd protein is transported in axons. (A) Wnd protein accumulates in segmental nerves when axonal transport is disrupted. Segmental nerves from third instar larvae are stained for axonal membrane (anti-HRP) in red and Wnd (anti-Wnd) in green. (right) Wnd staining alone is shown. (B) Wnd protein (top, green; bottom, alone) accumulates at the ligation (injury) site (blue rectangle). (C) Live imaging indicates that Wnd particles rapidly translocate both anterogradely and retrogradely in axons (Video 1). The GFP-wnd^KD transgene was expressed in motoneurons using the OK6-Gal4 driver. (top) A single 0.5-s exposure is shown. (bottom) A representative kymograph from a single axon is shown. Bars, 10 µm.