The nuclear and cell body response to axonal injury specifically requires Wnd and downstream signaling components. (A–D) puc-lacZ expression (left) and staining for DVGLUT (right) in VNCs uninjured and 24 h after injury. (A) The nerve crush injury induces an increase in puc-lacZ expression (left) and decrease in staining for DVGLUT (right) in motoneuron cell bodies. (B) The response to injury requires Wnd function. No obvious change in puc-lacZ (left) and DVGLUT (right) is observed after injury when Wnd is disrupted. (C) The response to injury is inhibited by FosDN. (D) Overexpression (OE) of wnd in neurons is sufficient to activate the injury response, including induction of puc-lacZ and reduction in DVGLUT staining. (E and F) Quantification of the puc-lacZ expression level before (white bars) or 24 h after (black bars) injury in different genotypes. The BG380-Gal4 driver is used to drive expression of all UAS lines (wnd RNAi, JNKDN, FosDN, JunDN, and Slpr). P > 0.05 was not significant. **, P < 0.001; ***, P < 0.0001. Error bars indicate mean ± SEM. Bars, 25 µm.
