The BMI1 and RNF8 recruitment to sites of DNA damage are independent of each other. Cells were treated and stained as in Fig. 7 A. (A) RNF8 WT and RNF8 KO cells. (B) BMI1 WT and BMI1 KO. Survival curves of MEFs transfected with either control shRNA or one of two different specific shRNA. Cells were treated as in Fig. 7 C. (C) RNF8 KO cells transfected with one of two different BMI1 shRNA. (D) BMI1 KO cells transfected with one of two different RNF8 shRNA. The experiment was performed in triplicate. Error bars represent standard error from two independent experiments (n = 6). Bars, 5 µm.