Analysis of naturally occurring pVHL point mutants. (A) Position of each naturally occurring point mutation within pVHL₃₀ domains and its disease class. pVHL₁₉ results from an internal translational initiation site at methionine 54 (M54). (B) Distributions of per cell means of growth track and shrinkage speeds (10–15 RCC-4 cells per mutant). The light blue line indicates the median value of RCC-4 cells expressing wild-type pVHL₃₀, and the red line indicates the median value of RCC-4 cells expressing the control vector (Figs. 1 I and 2 D). The speeds distributions of individual MT growth and shrinkage events are presented in Fig. S4 B. Boxes indicate 25, 50 (median), and 75% quantiles; whiskers extend to 1.5× the interquartile range; red dots indicate outliers beyond this range. Notched boxes indicate uncertainty of the median. Boxes whose notches do not overlap indicate that the medians of the two clusters differ at the 5% significance level. (C) Scatter plot of shrinkage probability (probability of a switch from a growth to a shrinkage event) versus time spent in shrinkage, relative to the total duration of trajectories.