Atg9-GFP distribution in various atg mutants. The atg11Δ (MMY069; A and B), atg1Δ (MMY068; C and D), atg13Δ (MMY070; E and F), and atg14Δ (MMY071; G and H) strains were grown and processed as described in Fig. 2. Cryosections were immunolabeled for GFP alone (A, B, and D–J) or in combination with Ape1 (C). (A and B) The Atg9 reservoirs observed in atg11Δ cells. (C and D) The PAS accumulated in the atg1Δ knockout. (E and F) The PAS present in the atg13Δ strain displays a Cvt complex surrounded by numerous small vesicles. (G and H) Enrichment of tubular membranes at the PAS of the atg14Δ mutant. Cvt complexes are highlighted with broken lines. D–F are also shown in Fig. S4 (E, F, I, and J) without dashed lines for clarity, while additional examples are presented in Fig. S4 (A–D, G, H, and K–Q). CW, cell wall; M, mitochondria; N, nucleus; PM, plasma membrane; V, vacuole. Bars, 200 nm.