Overexpression of GFP-BazS980A disrupts epithelial morphogenesis and induces apoptosis. (a) A late stage embryo overexpressing GFP-BazS980A with da::GAL4 shows a severely disrupted morphology caused by the degeneration of the epidermis. The embryo is dorsally open, and internal organs including the foregut (arrow) and midgut (arrowhead) protrude to the outside. (b) Upon overexpression of GFP-BazS980A with da::GAL4, many epidermal cells die by apoptosis, marked by TUNEL labeling. GFP-BazS980A puncta correspond to aggregates such as those shown at higher magnification in Fig. 1 e. (c) The dominant-negative effect of GFP-BazS980A overexpression is cell autonomous. GFP-BazS980A was overexpressed under control of en::GAL4 in segmentally repeated stripes. Note that aPKC and Crb are lost from the plasma membrane in cells that overexpress GFP-BazS980A but localize to the cell outlines in the region between stripes. See also Fig. S2 and Videos 1 and 2. Bars: (a and b) 100 µm; (c) 50 µm.