Model of CLIC endocytosis. (A) (1) CLIC cargo, such as Thy-1, CD44, and myoferlin are concentrated within Flotillin-1 and cholesterol-enriched microdomains. (2) Actin and GRAF-1 drive the initial formation of the carriers, within 15 s. (3) Recruitment of dynamin, Rab11, and Rab5/EEA-1 complexes within 2 min provides the ability for these carriers to facilitate bulk membrane flow to early endosomes (4a) and fast plasma membrane recycling (4b). (B) After abrasion to the PM an influx of Ca²⁺ activates the fusogenic C2 domains of dysferlin/myoferlin, resulting in the preferential recycling of the CLIC pathway.