Figure 3.
Figure 3. 3D morphology of CLICs. (A) Cav1−/− MEFs were incubated with CTxB-HRP for 20 min on ice, before internalization for 15 s. The DAB reaction was performed and cells were processed for electron tomography (see Materials and methods). A single section of the original tomogram is shown (left). Various rotations of a 3D contoured electron density render were generated (middle). Enlarged sections selected from the tomogram (right) show internal vesicles (arrows) and a complete connection around the circumference of the structure (arrowhead). (B) WT MEFs grown on sapphire discs were incubated with CTxB-HRP for 15 s before DAB reaction and high-pressure freezing. Tubular extensions (large arrows) are seen emanating from vesicular bulbs (arrowheads) in the characteristic ring-shaped CLIC morphology. CCPs without CTxB label (double arrowheads) indicate that they are still surface connected. Bars, 200 nm.

3D morphology of CLICs. (A) Cav1−/− MEFs were incubated with CTxB-HRP for 20 min on ice, before internalization for 15 s. The DAB reaction was performed and cells were processed for electron tomography (see Materials and methods). A single section of the original tomogram is shown (left). Various rotations of a 3D contoured electron density render were generated (middle). Enlarged sections selected from the tomogram (right) show internal vesicles (arrows) and a complete connection around the circumference of the structure (arrowhead). (B) WT MEFs grown on sapphire discs were incubated with CTxB-HRP for 15 s before DAB reaction and high-pressure freezing. Tubular extensions (large arrows) are seen emanating from vesicular bulbs (arrowheads) in the characteristic ring-shaped CLIC morphology. CCPs without CTxB label (double arrowheads) indicate that they are still surface connected. Bars, 200 nm.

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