KIF17 participates in epithelial cystogenesis. (A) Immunolocalization of GP135, E-cadherin, and DAPI in control (shNC) and KIF17-depleted (shKIF17#1) MDCK cysts grown 2 d in Matrigel. (B) Quantification of 2–4 cell cysts with central, peripheral, and nonpolarized GP135 localization. (C) Immunolocalization of GP114, ZO-1, and DAPI in control and KIF17-depleted MDCK cysts grown 5 d in Matrigel. (D) Graph shows percentage of cysts with 0, 1, or >1 lumen. (E) Percentage of cysts with apically polarized GP114. All analyses are derived from three independent experiments; 100–200 cysts were analyzed per condition. (F) Tyrosinated tubulin, acetylated tubulin, and DAPI staining in mature control cyst. Error bars indicate SEM. (G) Proposed role for KIF17 in MT stabilization and epithelial polarization.