Figure 9.
Figure 9. KIF17 affects MT stabilization in epithelial cells. (A) Immunostaining of tyrosinated and acetylated tubulin in MDCK cells infected with shNC or shKIF17#1 and treated for 45 min with 33 µM NZ. (B) Acetylated and tyrosinated tubulin in MDCK cells (outlined cells and inset), treated for 1 h with 33 µM NZ. (C) Caco2 cells injected with GFP-KIF17G754E cDNA and treated for 15 min with 33 µM NZ. An enlargement of the boxed region is shown on the right. Arrows point to GFP-KIF17G754E at the ends of NZ-resistant MTs.

KIF17 affects MT stabilization in epithelial cells. (A) Immunostaining of tyrosinated and acetylated tubulin in MDCK cells infected with shNC or shKIF17#1 and treated for 45 min with 33 µM NZ. (B) Acetylated and tyrosinated tubulin in MDCK cells (outlined cells and inset), treated for 1 h with 33 µM NZ. (C) Caco2 cells injected with GFP-KIF17G754E cDNA and treated for 15 min with 33 µM NZ. An enlargement of the boxed region is shown on the right. Arrows point to GFP-KIF17G754E at the ends of NZ-resistant MTs.

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