KIF17 affects MT acetylation in epithelial cells. (A and B) Immunostaining of tyrosinated and acetylated tubulin in MDCK cells infected with control (shNC) or KIF17 (shKIF17#1) shRNA (A), or fixed 5 h after microinjection of GFP-KIF17^G754E cDNA (B). (C) Quantitative immunoblot analysis of acetylated tubulin in MDCK cells treated with control or KIF17 shRNA, or expressing GFP or GFP-KIF17^G754E. The ratio of acetylated tubulin/GAPDH was normalized to 1 in controls. Data were derived from 3–5 experiments. Error bars indicate SEM. (D) KIF17-depleted MDCK cells fixed 3 h after injection of GFP-KIF17^G754E cDNA and stained for tyrosinated and acetylated MTs. Outlined regions indicate injected cells. Insets show the injected GFP-KIF17^G754E.