KIF17 is necessary for APC localization at MT plus ends. (A) Immunostaining of endogenous APC (red) in MDCK cells fixed 1 d after transfection with GFP-KIF17-S (green). Arrows, APC clusters in cell protrusions in nonexpressing cells; arrowheads, protrusions lacking APC in a cell expressing GFP-KIF17-S. (B) Percentage of GFP-KIF17-S–expressing cells (n = 183) or surrounding controls (n = 189) with APC clusters in protrusions. Data were compiled from three experiments. Error bars indicate SEM. (C) Immunostaining of APC and MTs in MDCK cells expressing control (shNC) or KIF17 (shKIF17#1) shRNAs. Arrows, APC clusters localizing at MT ends in cell protrusions; arrowheads, cell protrusions lacking APC clusters at MT ends. Insets show enlarged views of the boxed regions. (D) Rescue of APC localization in cell protrusions by expression of GFP-KIF17G754E in KIF17-depleted MDCK cells. Boxed regions are shown at high magnification on the right.
