KIF17 coiled-coil region binds APC independently of EB1. (A) Colocalization of GFP-fAPC and mRFP-KIF17G754E at plus ends of a subset of MTs in Caco2 cells 3 h after cDNA injection. The boxed region is magnified on the right. (B) Immunostaining of APC and tyrosinated tubulin in MDCK cells expressing GFP-KIF17G754E. Color overlays (a and b) show magnifications of the boxed regions. (C) Schematic of wild-type (WT) and C-terminally truncated APC present in Caco2 cells. The red arrowhead shows the EB1 binding site, which is missing in Caco2 APC. Immunoblots show endogenous APC, EB1, KIF17, and KIF3 in total lysates (TL) and immunoprecipitates from Caco2 cells incubated with rabbit IgG or anti-APC IgG. Numbers on top indicate amino acid position. (D) Immunoblots showing total lysates and immunoprecipitated APC (endogenous) and GFP-KIF17 constructs expressed in Caco2 cells.
