KIF17 motor domain interacts with EB1. (A) Immunoprecipitation of endogenous KIF17 or KIF3 from HEK-293 cells (AP, affinity purified IgG). Western blots probed for KIF17, KIF3A, or EB1. 1/20 volume used for immunoprecipitation was loaded for total lysates. At this concentration, KIF17 is not detected. (B) Schematic diagram of EB1 and KIF17 constructs. Numbers indicate amino acid position. (C) Immunoprecipitation of myc-tagged KIF17-FL, KIF17-M, or KIF17-T from MDCK cells cotransfected with GFP-tagged EB1-FL, EB1-N, or EB1-C. Immunoprecipitates and total lysates were blotted with anti-myc and anti-GFP antibodies. (D) Binding of in vitro translated HA-KIF17-FL to purified GST-tagged EB1-FL (FL), EB1-C (C), or EB1-N (N). TL, total lysate. (E) Pull-down of GFP-tagged EB1 constructs expressed in MDCK cells by purified GST-KIF17-M. (F) Coimmunoprecipitation of myc-EB1-FL and GFP-KIF17-M, GFP-KIF17-M^IP, or GFP-KIF17-MΔ5 from MDCK lysates.