Mutants that interfere with the oscillatory behavior. (a and b) GAL-CLB2dBΔ cdc14-1 (1575) cells were arrested with α-factor in YEPR and released into medium supplemented with 1 (a) or 0.05% (b) galactose to induce the expression of Clb2. (a) The percentages of cells with metaphase spindles (closed squares), anaphase spindles (closed circles), and cells with Cdc14 released from the nucleolus (open circles) were determined at the indicated times. Arrows indicate the time of the temperature shift (180 min). (c and d) GAL-CLB2dBΔ CDC5dBΔ (Ry1392) cells were treated as in a and b, respectively. (e and f) GAL-CLB2dBΔ cdh1Δ (Ry1466) cells were treated as in a and b, respectively. (c–f) Cell samples were analyzed as in a.