Deletion of the TORC2 component PiaA suppresses the prolonged phosphorylation of PKBR1 and PKB substrates caused by RasC^Q62L. (A–C) Flag-RasC or -RasC^Q62L was expressed under the control of a doxycycline-inducible promoter in wild-type (WT; A), piaA⁻ (B), or aca⁻ cells (C). Cells were stimulated with cAMP, sampled at the indicated time points, and probed with phospho-specific antibodies or anti-Flag antibody. The protein-transferred membrane was stained with Coomassie brilliant blue (CBB) and shown as loading control. Vertical black lines indicate that intervening lanes have been spliced out.