Super-resolution microscopy of GTP-tubulin clusters and KIF5 binding sites in axonal MTs of stage 4 hippocampal neurons. (A, A′, and B) Reconstructed super-resolution microscopy images of hMB11 staining (A and A′) and anti–α-tubulin antibody staining (B) followed by ATTO565-labeled secondary antibodies. A′ shows a magnification of the boxed area in A. Note that hMB11 staining was more abundant in axons (arrows), whereas α-tubulin staining was not polarized. Bars, 25 µm. (C and D) Motor domain binding sites in axons. KIF5 binding sites (C) and KIF1A binding sites (D). Dronpa-fused motors (green) and hMB11 staining (red) were observed. Colocalization is indicated by asterisks. Bars, 2 µm.