KIF5 motor head domain for axonal preference. Chimeric proteins of KIF1A (C381) and KIF5C (K381) were expressed in stage 3 neurons. Neurons were classified based on the numbers of neurites that accumulated the chimera at their tips, and their percentages among all neurons are shown in the graph. One tip corresponds to the selective accumulation in axons. Bottom panels show representative images of neurons expressing each construct. YFP images were superimposed on differential interference contrast microscopy images. Arrows indicate the tips of axons and asterisks indicate the cell bodies of transfected neurons. Bars, 10 µm.