Staufen protein is transported on MTs at the oocyte posterior. Also see Fig. S1 and Video 4. (A) Dual-channel imaging of Staufen-RFP (A, left and bottom) and Tau-GFP (A, right and bottom). Dashed arrows indicate paths of cargo transit along the cortex. (B) A single Staufen particle moving on Tau-GFP–labeled MTs taken from the outlined region in A (bottom). (B, bottom) Time sequence of the particle (right, white arrowheads) moving in two runs along two different MTs (left, black arrowheads). The two MTs are indicated with arrowheads in the last three images to highlight the depolymerization of the lower one. A, anterior; P, posterior; N, oocyte nucleus. Bars: (A and C) 15 µm; (B) 7.5 µm.