EB1 tracks the plus ends of dynamic MTs throughout the oocyte. (A) Dual-channel imaging of Tau-GFP and EB1-mCherry expressed in the same oocyte reveals the association of EB1 with the plus ends of Tau-labeled MTs throughout the oocyte (Video 2). Note that the density of EB1 tracks matches the gradient in MT density from the anterior to posterior of the oocyte and that individual MTs extend well into the extreme posterior. Insets show the portion of the oocyte imaged and its orientation. (B) Tau-GFP and EB1-GFP expressed in the same oocyte allow individual extending MTs to be followed (Video 3). (right) Detail of two individual MTs extending (black arrowheads mark the length of the MT, and white arrowheads mark the extending plus end) taken from the region shown in B (bottom dashed box and white arrowheads). A series of four time points is shown. (C) A time course sequence from a time-lapse video showing that at the extreme posterior, MTs tend to bend round the cortex back on themselves. The images correspond to the top large region in B outlined with white dots. White arrowheads highlight a single extending MT, whereas at the final time point, several MTs are indicated with dashed arrows, which is drawn from Video 3. A, anterior; P, posterior. Bars: (A and B, left) 15 µm; (B, right; and C) 7.5 µm.