Figure 1.

A dynamic network of MTs extends throughout the oocyte posterior. Also see Video 1. (A–A′′) Tau-GFP–labeled MT in a living stage 9 oocyte. (A) Overview from anterior to posterior (projected 10-µm z series at reduced magnification) showing the gradient in MT density. (A′ and A′′) Two regions at increased magnification (100× 1.4 NA oil objective) showing the tight dense network of MTs toward the anterior and the more sparse network of MTs extending into the extreme posterior (projected 4-µm z series, which were contrasted individually to display the MT present). (B) Comparison of the Tau-GFP–labeled MT distribution at three time points merged as an RGB image to highlight the changes. The individual channels are shown below. Inserted diagrams in A and B show the orientation of the Drosophila egg chamber and the portion of the oocyte imaged. Throughout the images, the convention is posterior to the right. Bars: (A) 25 µm; (A′ and B) 10 µm.

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