Figure 7.
Figure 7. The CPC localizes to different spindle subdomains in response to Sli15/INCENP phosphorylation by Ipl1/Aurora and Cdk1. (A) Ipl1 inhibition partially restores CPC localization to the anaphase spindle in cdc14-2 cells. Localization of Sli15-3GFP and Tub1-mCherry in IPL1 cdc14-2 or ipl1-as6 cdc14-2 cells exposed to the restrictive temperature (34°C) for 2 h and then treated with 60 µM 3-MB-PP1 for 15 min. Bar, 2 µm. (B) Quantification of observations represented in A; error bars show standard errors. (C) sli15-17A partially restored its localization to the anaphase spindle in cdc14-2 cells. Localization of Sli15-3GFP or sli15-17A-3GFP and Tub1-mCherry in cdc14-2 strains exposed to 34°C for 2 h. Bar, 2 µm. (D) Quantification of observations represented in C; error bars show standard errors.

The CPC localizes to different spindle subdomains in response to Sli15/INCENP phosphorylation by Ipl1/Aurora and Cdk1. (A) Ipl1 inhibition partially restores CPC localization to the anaphase spindle in cdc14-2 cells. Localization of Sli15-3GFP and Tub1-mCherry in IPL1 cdc14-2 or ipl1-as6 cdc14-2 cells exposed to the restrictive temperature (34°C) for 2 h and then treated with 60 µM 3-MB-PP1 for 15 min. Bar, 2 µm. (B) Quantification of observations represented in A; error bars show standard errors. (C) sli15-17A partially restored its localization to the anaphase spindle in cdc14-2 cells. Localization of Sli15-3GFP or sli15-17A-3GFP and Tub1-mCherry in cdc14-2 strains exposed to 34°C for 2 h. Bar, 2 µm. (D) Quantification of observations represented in C; error bars show standard errors.

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