Figure 3.
Figure 3. Ectopic localization of active Ipl1/Aurora to metaphase spindles decreases MT dynamics. (A) sli15-17A increases centromere mispositioning in metaphase. Percentage of cells showing different classes of CEN15 (marked with GFP) positioning relative to Spc42-GFP in SLI15 and sli15-17A cells. (B) sli15-17A reduces MT polymerization in metaphase. Representative FRAP images of GFP-Tub1–marked spindles in SLI15 and sli15-17A cells. Photobleached areas are indicated with squares (at 3 s). (C) FRAP quantification of B reveals decreased MT dynamics in sli15-17A cells. (D) Ipl1 inhibition has no statistically significant effect on MT dynamics. FRAP quantification for metaphase spindles from IPL1 and ipl1-as6 cells treated with 40 µM 3-MB-PP1 for 15 min. (E) Ipl1 inhibition alleviates the decreased MT polymerization caused by sli15-17A. FRAP quantification for metaphase spindles from SLI15 ipl1-as6 and sli15-17A ipl1-as6 cells treated with 40 µM 3-MB-PP1.

Ectopic localization of active Ipl1/Aurora to metaphase spindles decreases MT dynamics. (A) sli15-17A increases centromere mispositioning in metaphase. Percentage of cells showing different classes of CEN15 (marked with GFP) positioning relative to Spc42-GFP in SLI15 and sli15-17A cells. (B) sli15-17A reduces MT polymerization in metaphase. Representative FRAP images of GFP-Tub1–marked spindles in SLI15 and sli15-17A cells. Photobleached areas are indicated with squares (at 3 s). (C) FRAP quantification of B reveals decreased MT dynamics in sli15-17A cells. (D) Ipl1 inhibition has no statistically significant effect on MT dynamics. FRAP quantification for metaphase spindles from IPL1 and ipl1-as6 cells treated with 40 µM 3-MB-PP1 for 15 min. (E) Ipl1 inhibition alleviates the decreased MT polymerization caused by sli15-17A. FRAP quantification for metaphase spindles from SLI15 ipl1-as6 and sli15-17A ipl1-as6 cells treated with 40 µM 3-MB-PP1.

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