Figure 1.
Figure 1. The CPC has a diffuse nuclear localization in preanaphase that requires MTs. (A, top) MTs regulate preanaphase CPC localization. Representative cells containing BIR1-3GFP treated with DMSO or nocodazole (NOC). Bar, 2 µm. (bottom) Time-lapse Bir1-3GFP images from Video 1 for the cells above. (B) CPC localizes to chromatin arms as well as centromeres in preanaphase. PCR products amplified from DNA obtained by chromatin immunoprecipitation from cells containing BIR1-13myc (left) and primer pair locations (right). IN, input DNA; −, no antibody; +, anti-myc antibody.

The CPC has a diffuse nuclear localization in preanaphase that requires MTs. (A, top) MTs regulate preanaphase CPC localization. Representative cells containing BIR1-3GFP treated with DMSO or nocodazole (NOC). Bar, 2 µm. (bottom) Time-lapse Bir1-3GFP images from Video 1 for the cells above. (B) CPC localizes to chromatin arms as well as centromeres in preanaphase. PCR products amplified from DNA obtained by chromatin immunoprecipitation from cells containing BIR1-13myc (left) and primer pair locations (right). IN, input DNA; −, no antibody; +, anti-myc antibody.

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