Ectopic Cdc14 activation does not cause premature degradation of the meiotic cyclin Clb1. (A–D) Wild-type (AM6770; A), cdc14-1 (AM7815; B), cdc55mn (AM6961; C), and cdc14-1 cdc55mn (AM7816; D) cells carrying CLB1-9MYC, GAL-NDT80, and pGPD1-GAL4(848).ER were released from a pachytene arrest. The percentages of cells with the indicated spindle morphology (top) or Clb1-9Myc localization (middle) were determined at the indicated time points. The anti-Myc immunoblot (bottom) is shown with the anti-Pgk1 immunoblot as a loading control and the positions of molecular mass markers indicated. Western blots are representative (A and C) or were performed once (B and D). Numbers at the top of the blots indicate time in hours.