The cdc14-1 mutation rescues homologue segregation in cdc55mn cells but does not prevent equational segregation on the same axis. (A) Segregation of homozygous CEN5-GFP foci in ≥1,000 binucleate cells of wild-type (AM6040), cdc55mn (AM5936), cdc14-1 (AM6902), and cdc14-1 cdc55mn (AM6908) strains were determined as described in Fig. 1 D. (B–F) Segregation of heterozygous CEN5-GFP in wild-type (AM4796), cdc55mn (AM4891), cdc14-1 (AM6910), and cdc14-1 cdc55mn (AM6934) cells. (B) Segregation in binucleate cells was determined as described in A. (C–F) The total percentage of binucleate and tetranucleate cells and the percentage of binucleate cells that have GFP foci in one, both, or between the two nuclei are shown for a representative experiment.