Figure 2.
Figure 2. Impaired spindle assembly in cdc55mn cells is caused by ectopic Cdc14 activation. (A–F) Wild-type (AM6142; A), cdc55mn (AM6131; B), cdc14-1 (AM7549; C), cdc14-1 cdc55mn (AM7550; D), mad2Δ (AM7547; E), and mad2Δ cdc55mn (AM7548; F) strains carrying REC8-3HA, PDS1-18MYC, GAL-NDT80, and pGPD1-GAL4(848).ER were cultured as described in Fig 1 (F and G). The percentages of binucleate and tetranucleate cells, Pds1-positive cells, and cells with the indicated spindle morphology were determined. Anti-HA and anti-Myc immunoblots showing the positions of full-length Rec8-3HA, cleaved Rec8-3HA, and Pds1-18Myc (arrowheads) are shown with protein molecular mass markers in black (anti-HA) or gray (anti-Myc). Arrows indicate a reduction in Pds1-positive cells in the first and second meiotic division. Blots are representative (A and B) or were performed once (C–F). Numbers at the top of the blots indicate time in hours.

Impaired spindle assembly in cdc55mn cells is caused by ectopic Cdc14 activation. (A–F) Wild-type (AM6142; A), cdc55mn (AM6131; B), cdc14-1 (AM7549; C), cdc14-1 cdc55mn (AM7550; D), mad2Δ (AM7547; E), and mad2Δ cdc55mn (AM7548; F) strains carrying REC8-3HA, PDS1-18MYC, GAL-NDT80, and pGPD1-GAL4(848).ER were cultured as described in Fig 1 (F and G). The percentages of binucleate and tetranucleate cells, Pds1-positive cells, and cells with the indicated spindle morphology were determined. Anti-HA and anti-Myc immunoblots showing the positions of full-length Rec8-3HA, cleaved Rec8-3HA, and Pds1-18Myc (arrowheads) are shown with protein molecular mass markers in black (anti-HA) or gray (anti-Myc). Arrows indicate a reduction in Pds1-positive cells in the first and second meiotic division. Blots are representative (A and B) or were performed once (C–F). Numbers at the top of the blots indicate time in hours.

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