Figure 3.
Figure 3. In NUDF-depleted cells, p25 is required for the localization of dynein (GFP-HC) and dynactin (p150-GFP) to the hyphal tip region, where early endosomes are enriched. (A) Images showing that in the same ΔnudF cells, both GFP-HC and mCherry-RabA localize to the hyphal tip region. (B–D) In NUDF-depleted cells (alcA-nudF grown on glucose), p25 is required for the localization of dynein and dynactin to the hyphal tip region where early endosomes are found. Images of GFP-HC (B), p150-GFP (C), and GFP-RabA (D) are shown. In the absence of NUDF (alcA-nudF), a portion of GFP-HC and p150-GFP proteins localize to the hyphal-tip region, where early endosomes also accumulate (B–D, middle). Introducing the Δp25 mutant allele into this background (alcA-nudF/Δp25) abolishes this hyphal tip accumulation but maintains the plus-end comets of GFP-HC and p150-GFP (B and C, right). Bars, 5 µm.

In NUDF-depleted cells, p25 is required for the localization of dynein (GFP-HC) and dynactin (p150-GFP) to the hyphal tip region, where early endosomes are enriched. (A) Images showing that in the same ΔnudF cells, both GFP-HC and mCherry-RabA localize to the hyphal tip region. (B–D) In NUDF-depleted cells (alcA-nudF grown on glucose), p25 is required for the localization of dynein and dynactin to the hyphal tip region where early endosomes are found. Images of GFP-HC (B), p150-GFP (C), and GFP-RabA (D) are shown. In the absence of NUDF (alcA-nudF), a portion of GFP-HC and p150-GFP proteins localize to the hyphal-tip region, where early endosomes also accumulate (B–D, middle). Introducing the Δp25 mutant allele into this background (alcA-nudF/Δp25) abolishes this hyphal tip accumulation but maintains the plus-end comets of GFP-HC and p150-GFP (B and C, right). Bars, 5 µm.

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